|
Tissue culture |
Growth area (cm2) |
Medium volume (ml) |
*1Xtrypsin or |
|
Microwell plates |
|
|
|
|
96 well |
0.32 |
0.3 |
0.3 |
|
24 well |
2.0 |
0.5 - 1.0 |
0.5 |
|
12 well |
3.8 |
1 - 2 |
1 |
|
6 well |
9.6 |
2 |
1 |
|
Culture Dishes |
|
|
|
|
35 mm |
9.6 |
2 |
1 |
|
60 mm |
21 |
5 |
2 |
|
100 mm |
55 |
10 |
5 |
|
150 mm |
145 |
20-30 |
8 |
|
Flasks |
|
|
|
|
25 cm2 (T-25) |
25 |
5-10 |
2 |
|
75 cm2 (T-75) |
75 |
15-30 |
5 |
|
175 cm2 (T-175) |
175 |
30-50 |
8 |
|
225 cm2 (T-225) |
225 |
50-70 |
15 |
|
Roller Bottles |
|
| |
|
1250 ml |
490 |
100-200 |
- |
|
2200 ml |
850 |
100-250 |
- |
|
4900 ml |
1750 |
100-500 |
- |
* 1X trypsin: contains 0.25% porcine trypsin (1:250)
* 1X trypsin-EDTA: contains 0.05% porcine trypsin (1:250) and 0.53 mM EDTA·4Na
